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sample submission

Sanger Sequencing

 Sample TypePCR Product
 Tube0.2ml tube / strip
 Volume20 µL 
 Concentration20-50 ng/μL 
 LabelingClearly label the tubes with unique identifiers that should match your sample request form
 Shipment & PackagingPlace the tubes in zip lock bags before packing in styroform box with 2 to 3  frozen gel packs
   

Sample Primers

  
 Provide forward & reverse sequence so we can check your primer compatibility  
 Primer Design Features 
 GC content between 50 to 64 
 Self complementarity < 4       
 TM between 50° to 64° 
 Target Length shouldn’t be less than 300bp 
 If primers are optimized provide annealing temperature. 
 Required primer concentration is 10 µM & volume 5 μL/sample